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Med J Tabriz Uni Med Sciences. 2024;46(1): 19-28.
doi: 10.34172/mj.2024.010
  Abstract View: 100
  PDF Download: 39

Immunology and Allergy

Original Article

Hsa-Mir-1 and Hsa-Mir-1229 expression levels in recurrent miscarriage patients before and after lymphocyte therapy in comparison to control groups

Mortaza Raeisi 1 ORCID logo, Mehdi Yousefi 2 ORCID logo, Javad Ahmadian Haris 3 ORCID logo, Shahla Danaii 4, Mohammad Tofighifard 5, Pouya Bahlouli 5, leili Aghebati-Maleki 6* ORCID logo

1 Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
2 Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
3 Department of Pediatric Diseases, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4 Gynecology Department, Eastern Azerbaijan ACECR ART Center, Eastern Azerbaijan Branch of ACECR, Tabriz, Iran
5 Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Email: leili_aghebati_maleki@yahoo.com

Abstract

Background. Recurrent pregnancy loss is defined as at least two consecutive clinical pregnancy losses before 20th week of gestation. In this study, we investigated microRNA-1 and microRNA-1229 in recurrent miscarriage patients before and after lymphocyte therapy in comparison to control group.

Methods. First, a written and informed consent will be obtained from the target population of this study, who are people with RPL referring to Waliasr International Hospital. According to the standard protocol, the number of 2×107 is injected subcutaneously to patients. Real-time PCR technique will be used to check the expression level of microRNA-1 and microRNA-1229.

Results. The gene expression level of micro-RNA 1 in RPL women before of lymphocyte therapy was 2.030±1.445 and the gene expression level of the same micro-RNA after the lymphocyte therapy of the mother was 1.101±0.4780. Also, the expression level of micro-RNA 1229 in these women was reported to be 2.100±0.6296 before treatment and 1.247±0.9631 after treatment with lymphocytes. This level of gene expression in control group, for micro-RNA 1 and micro-RNA 1229 genes is 1.000±0.08334 and 1.000±0.08091, respectively.

Conclusion. The results of our study showed a noticeable decrease in the gene expression of microRNAs under study, both microRNAs 1 and 1229, in women with a history of three consecutive injections of the father's subcutaneous lymphocytes.

Practical Implications. This study can show the therapeutic effect of lymphocyte for patients with recurrent miscarriage so that this treatment can be used along with other treatment methods.


How to cite this article: Raeisi M, Yousefi M, Ahmadian Haris J, Danaii Sh, Tofighifard M, Bahlouli P, Aghebati- Maleki L. Hsa-miR-1 and Hsa-miR-1229 Expression Levels in Recurrent Miscarriage Patients Before and After Lymphocyte Therapy in Comparison to Control Groups. Med J Tabriz Uni Med Sciences. 2024;46(1):19-28. doi: 10.34172/mj.2024.010. Persian.
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Submitted: 31 Jul 2023
Accepted: 23 Sep 2023
ePublished: 26 Feb 2024
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