Med J Tabriz Uni Med Sciences Health Services. 2014;36(4): 10-13.
  Abstract View: 514
  PDF Download: 103

Original Article

The Effect of Plgf Gene Knockdown on Enzymes Metalloproteinase-2 and -9 (MMP-2&-9) Activity

Hassan Akrami 1, Mansour Aminzadeh 1, Ameneh Sharifi 2*

1 Department of Biology, School of Science, Razi University, Kermanshah, Iran
2 Marital Counseling Center, Ilam University of Medical Sciences, Ilam, Iran
*Corresponding Author: Email: A_sharifi_genetic@yahoo.com


Background and Objectives: Placental Growth Factor (PlGF) is a member of Vascular Endothelial Growth Factors (VEGF) family, which is involved in several pathologic processes, including the growth and spread of malignancies. PlGF promote cancer metastasis by controlling the MMP (Matrix metalloproteinase) activity. In the present study, we investigate the correlation between the expression of PlGF and matrix metalloproteinase enzymes activity in human Adenocarcinoma Gastric Cell (AGC). Methods and Materials: Human AGC were treated with 20, 40 and 80 picomol concentrations small interfering RNA (siRNA) related to PlGF mRNA for24, 48 and 72 hours. Then MMP-2 and MMP-9 enzymatic activity was measured by gelatin zymography test. Results: The gelatin zymography results indicated that the activity of MMP2&9 was fully inhibited in AGC which were treated with various concentrations of PlGF-siRNA for 24 hours. Our results also showed that in cells were treated with PlGF-siRNA for 48 hours the activity of MMP2 and MMP 9 were decreased (but not fully inhibited). Conclusion: According to this study reduction of PlGF gene expression may prevents MMP2/9 activity in AGC. These data suggest that PlGF may function up stream of MMP in gastric adenocarcinoma cells. Probably PlGF effect on cancer cell metastasis via regulation activity of MMPs.
First Name
Last Name
Email Address
Security code

Abstract View: 515

Your browser does not support the canvas element.

PDF Download: 103

Your browser does not support the canvas element.

Submitted: 24 Sep 2013
Accepted: 15 Dec 2013
ePublished: 29 Oct 2014
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)