The In Vitro Study of Comparison of Scolicidal Effectiveness of Physical Agents (Temperature and Smolarity), Chemical Agent (Albendazole) and Aquatic Extract of Harmel (Peganum Harmala L)

Abstract

Background and Objectives: Finding an effective scolicide in treatment of hydatic cyst is an important fact. The present study was done to compare the effect of scolicides such as physical agents (temperature and smolarity), chemical agents (Albendazole) and aquatic extract of Harmel (Peganum harmala) in vitro. Material and Methods: In this study, protoscolices were collected aseptically from sheep livers and lungs affected with hydatid cyst in Tabriz slaughterhouse. In laboratory, viability of protoscolices was determined by 0.1% Eosin staining. Scolicidal effect of 20%Nacl, 10% Nacl, 50% Dextrose, 2% Albendazol, temperature( -20oC, 40oC, 60oC ) and various dilutions of Harmel aquatic extract ( 25%, 50%, 75% , 100%) were evaluated after 5, 10, 15 and 60 min. The results were analyzed by Duncan and Tucky tests. Results: Twenty percent of Nacl after 60 min has significantly scolicidal effect than other agents (P<0.05), but it eliminated only 48.5% of protoscolices. Conclusion: The effect of 20% Nacl was better than physical, Chemical and plant agents, but long time for effecting limit using about it in surgery.

Keywords: Scolicidal, Albendazole, Aquatic extract of Peganum harmala L, In Vitro.