Study of hypericum perforatum hydroethanolic leaf’s extract on anemia & serum iron in male rats induced with cyclophosphamide

Abstract

Background: Cyclophosphamid is one of the anticancer drugs which cause alkylation on DNA in cells. These drugs reduce blood cells and affect bone marrow tissue. Hpericum perforatum (HP) is a medicinal plant which its hemopoetic effects on bone marrow function and blood parameter were investigated in this study. Methods: The 42 male Wistar rats with 220-250 gr body weight were divided in 6 groups randomly(n=7) : control group, treated group by cyclophosphamide(15mg/kg,i.p) , treated with HP, and treated groups (cyclophosphamide 15mg/kg, i.p, + 100 mg/kg HP, cyclophosphamide 15mg/kg, i.p, + 200 mg/kg HP and cyclophosphamide 15mg/kg, i.p, + 400 mg/kg HP). After the examination the blood samples were collected from heart directly and analyzed for RBC, HCT and Hb and Fe. Statistical analysis Results of this study were performed using SPSS statistical software, All data were expressed as mean±SEM and differences were considered statistically significant with P<0.05. Results: The bone marrow tissue was injured by cyclophosphamide with reduction in number of blood cells and increase blood iron significantly. The extract of HP showed that the treated animals have increase in blood cells and reduced blood iron compared with cyclophosphamide induced group significantly (P<0.001). Conclusion: The results showed that the extracts of Hypericum perforatum can bone marrow protect against the deleterious effects of Cyclophosphamide, and the blood parameters and serum iron close to normal levels.