Abstract
Background. Infection with human papillomavirus is the most critical risk factor for cervical cancer. Given the lack of detection of this virus type by serological methods, using PCR-based molecular methods is particularly important in the accurate and early detection of this virus. The present study aimed to develop and validate a sensitive and specific method based on TaqMan Real-time PCR for detecting HPV 31 and 33 genotypes using the E6 gene sequence.
Methods. In this descriptive-analytical study, 50 Pap smears of patients with cervical cancer were examined. After genomic DNA extraction, two high-risk genotypes, 31 and 33, were detected using the Multiplex Taqman Real-Time PCR method based on the E6 gene sequence.
Results. This study showed that 28% of the samples were positive for 31 and 33 genotypes. The frequency of infection with the 33 genotype was 22%, and the 31 genotype was 6%. Also, simultaneous infection with both genotypes was observed in 2% of samples. Data analysis showed a sensitivity of 97% and a specificity of 100%.
Conclusion. The Taqman Multiplex Real-Time PCR is an efficient and cost-effective method for screening 31 and 33 HPV genotypes. Due to its high accuracy, this method can be used for the early detection of HPV genotypes.
Practical Implications. Due to its high speed and accuracy, this technique can be suitable and cost-effective for identifying human HPV genotypes.