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Med J Tabriz Uni Med Sciences. 2021;43: 400-409.
doi: 10.34172/mj.2021.066
  Abstract View: 154
  PDF Download: 110

Original Article

Study of common chromosomal abnormalities and Plasminogen activator Inhibitor(PAI-1) gene Polymorphism (5G/4G) and Prothrombin (F2)gene mutation(G20210A) in women with recurrent abortions in the North West of Iran

Roya Bagheri 1 ORCID logo, Seyed ali Rahmani 2* ORCID logo, Leila Khoramifar 2 ORCID logo, Solmaz Ilkhichoui 2 ORCID logo

1 Faculty of Basic Sciences, Rabe-e Rashid Higher Education Institute, Tabriz, Iran
2 Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Email: rahmaniseyedali@yahoo.com

Abstract

Background. Parental chromosomal abnormalities as well as changes in genes encoding thrombophilic factors are common causes of recurrent abortions. One of the causes of thrombophilia is Factor II (F2) gene mutation (G20210A) and plasminogen activator inhibitor gene (PAI-1) polymorphism (4G/5G). Therefore, the present study aimed to investigate the frequency of chromosomal abnormalities and the association of thrombophilic gene polymorphisms in patients with abortion in northwestern Iran.

Methods. In the present case-control study, cytogenetic analysis of 60 couples with a history of recurrent abortions was performed by the standard 72-hour culture of lymphocytes and G-banding. The polymorphism (5G/4G) of PAI-1 gene and the mutation (G20210A) of F2 genes were evaluated using RFLP-PCR and ARMS-PCR molecular methods, respectively. The obtained data were analyzed using statistical software.

Results. No significant abnormalities affecting abortion were observed in cytogenetic studies; in the molecular study, the frequency of the 4G allele in patients and control groups were 54.2% and 33.3%, respectively; the frequency of 5G allele in the patients and control groups were 45.8% and 66.8%, respectively. The frequency of 5G/5G and 4G/5G genotypes is 25.0% and 41.6% in the patient group, and 55.0% and 23.3% in the control group, respectively. Also, the frequency of 4G/4G genotype in patients and controls were 33.3% and 21.6%, respectively.

Conclusion. The results of this study show that there is a significant relationship between the frequency of the 4G allele of the PAI-1 gene with susceptibility to recurrent abortions in northwestern women, while no was relationship between F2 gene mutation and recurrent abortions was observed.

Keywords: Recurrent Miscarriage, Plasminogen Activator Inhibitor-1, Chromosomal Abnormalities, Factor 2 Gene

How to cite this article: Bagheri R , Rahmani S.A, Khorramifar L, Ilkhchoui S. Study of common chromosomal abnormalities and plasminogen activator inhibitor-1 (PAI-1) gene polymorphism (5G/4G) and prothrombin (F2) gene mutation (G20210A) in women with recurrent abortions in North West of Iran. Med J Tabriz Uni Med Sciences. 2021. doi: 10.34172/mj.2021.066. Persian.
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Submitted: 31 Aug 2020
Revision: 23 Dec 2020
Accepted: 23 Dec 2020
ePublished: 03 Nov 2021
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