Study of IMP Type Β-Lactamase Gene’s Frequency in Escherichia Coli Isolates Collected from Patients at Educational Hospital (Imam Reza and Shohada), Tabriz, 2013

Abstract

Background & Objectives: Antibiotic resistance is one of the most problems in microbial infection control. Production of β-lactamases can lead to resistance against the third generation cephalosporins and carbapenems. This study was conducted to evaluate the antibiotic sensitivity profiles and the presence of blaIMP gene in Escherichia coli isolates, collected from clinical specimens in educational hospitals of Imam Reza and Shohada in Tabriz. Materials and Methods: In this study 100 Escherichia coli were isolated. These isolates collected from patients in educational hospital (Imam Reza and Shohada). They were identified by using conventional bacteriologic tests. Antimicrobial susceptibility test was performed according to Kirby- Bauer method. Confirmatory test for production of metallo- β-lactamase was also performed by using Modified Hodge test (MHT). blaIMP gene was detected by using PCR technique. Results: Results of antibiotic susceptibility tests revealed that the resistance rate against imipenem, meropenem, ertapenem, gentamycin, ciprofloxacin, ceftazidime, nalidixic acid, ceftriaxone and cefepime were 3%, 3%,4%, 44%, 69%, 64%, 79%, 68% and 53%, respectively. Result of MHT test revealed that 3% (3) isolates were metallo-β-lactamase producers. PCR amplification revealed that 5% (5) of isolates carried blaIMP gene. Conclusion: According to the more than 60% resistance of isolates from medical educational centers to the third generation cephalosporins and fluoroquinolone. In this study, we pose a consumption of carbapenemes as an alternative for serious bacterial infections. Even limited carbapenemase inducing resistance must raise awareness among hospital infectious control staff.