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Med J Tabriz Uni Med Sciences Health Services. 2015;37(4): 54-59.
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  PDF Download: 136

Original Article

Isolation of Lactobacillus Acidophilus and Assessment for Its Antiviral Activity against Herpes Simplex Virus Type II

Mehdi Moazzami Goudarzi 1*, Mohammad Vali Nejhad 2

1 Department of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Microbiology, College of basic science, Jahroum Branch, Islamic Azad University, Jahroum, Iran
*Corresponding Author: Email: Moma1675@gmail.com

Abstract

Background and Objectives: High prevalence of urogenital infections among women and increasing emergence of antibiotic resistance organisms are a challenging element. It is reasonable to consider the probiotics for combating the urogenital infections. Materials and Methods: In this study, 100 vaginal samples were collected from normal individuals and the presence of lactobacillus acidophilus was verified using biochemical and molecular characterization. the antiviral effect of lactobacillus acidophilus supernatant as a wild type and lactobacillus acidophilus La-5 supernatant, as a standard strain against herpes simplex virus type II were investigated. Results: The supernatant of Lactibacillus acidophilus culture significantly reduced plaque formation by herpes simplex virus II. The same results were obtained for standard strain. The inhibitory effects of each two supernatants were independent of H202 or H+ secretion by candidate lactobacilli. Conclusion: lactobacillus acidophilus is able to inhibit plaque formation by herpes simplex type II, independent from H2O2 and H+ production and it is related to another active bi-products that should be investigate in future studies.
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Submitted: 03 Feb 2014
Accepted: 17 Apr 2014
ePublished: 28 Oct 2015
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