Med J Tabriz Uni Med Sciences Health Services. 2014;36(3): 10-17.
  Abstract View: 593
  PDF Download: 101

Original Article

Preventing Human Hemoglobin Glycation Via Bee Venom

Javad Behroozi 1, Adeleh Divsalar 1*, Ali Akbar Saboury 2, Mohammad Nabiuni 1

1 Department of Cell and Molecular Biology, School of Biological Sciences, Kharazmi University, Tehran, Iran
2 Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
*Corresponding Author: Email: Divsalar@khu.ac.ir


Background and Objectives: Diabetes mellitus (DM) is a metabolic disorder witch the Hyperglycemia as its common feature leads to increased glucose concentration in proteins surroundings. The result of hyperglycemia is non-enzymatic addition of sugars to proteins which called glycation. This reaction leads to structural and functional alterations in proteins surroundings. The aim of this study was to investigate the effect of Bee venom on preventing of the human hemoglobin glycation. Materials and Methods: In this experimental study, human hemoglobin was incubated in the presence and absence of glucose in different concentrations of Bee venom for five weeks in 37 C. The extent of free amino groups in hemoglobin structure, percent of β-sheets, soret band absorption and Heme degradation before and after incubation were examined using UV-visible spectroscopy and fluorometry methods. Results: Glycation leads to heme degradation and increasing in β-sheet content of glycated hemoglobin compared to the control hemoglobin. Also, glycation decreases absorption of soret band in hemoglobin. Bee venom inhibits the glycation and decreases structural alterations in human hemoglobin in a dose dependent manner. Conclusions: Bee venom has a significant antiglycation effect and it can be used as a natural drug for preventing DM complications.
First Name
Last Name
Email Address
Security code

Abstract View: 594

Your browser does not support the canvas element.

PDF Download: 101

Your browser does not support the canvas element.

Submitted: 12 May 2013
Accepted: 06 Jul 2013
ePublished: 27 Aug 2014
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)