Preventing Human Hemoglobin Glycation Via Bee Venom

Abstract

Background and Objectives: Diabetes mellitus (DM) is a metabolic disorder witch the Hyperglycemia as its common feature leads to increased glucose concentration in proteins surroundings. The result of hyperglycemia is non-enzymatic addition of sugars to proteins which called glycation. This reaction leads to structural and functional alterations in proteins surroundings. The aim of this study was to investigate the effect of Bee venom on preventing of the human hemoglobin glycation. Materials and Methods: In this experimental study, human hemoglobin was incubated in the presence and absence of glucose in different concentrations of Bee venom for five weeks in 37 C. The extent of free amino groups in hemoglobin structure, percent of β-sheets, soret band absorption and Heme degradation before and after incubation were examined using UV-visible spectroscopy and fluorometry methods. Results: Glycation leads to heme degradation and increasing in β-sheet content of glycated hemoglobin compared to the control hemoglobin. Also, glycation decreases absorption of soret band in hemoglobin. Bee venom inhibits the glycation and decreases structural alterations in human hemoglobin in a dose dependent manner. Conclusions: Bee venom has a significant antiglycation effect and it can be used as a natural drug for preventing DM complications.

Keywords: Diabetes metabolic, Glycation, Bee venom, Hemoglobin