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Med J Tabriz Uni Med Sciences Health Services. 2014;36(1): 74-81.
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Original Article

Effects of Hydroethanolic Extract of Elaeagnus angustifolia on Pentylenetetrazole Induced Seizure in Male Mice

Naser Mirazi*, Abdolkarim Hosseini

1 Department of Biology, School of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran
*Corresponding Author: Email: mirazi205@gmail.com

Abstract

Background and Objectives: Epilepsy is a major cause of morbidity in patients. Elaeagnus angustifolia has antioxidant and anti-inflammatory activity. The effects of this traditional herbal medicine on Pentylenetetrazole (PTZ) -induced seizure have not been studied yet. The present study evaluated the effects of E. angustifolia on PTZ-induced seizure in male mice. Materials and Methods: 30 male mice (30±5 g) were randomly divided into 5 groups including: control, normal saline, phenobarbital and E. angustifolia’s extract group at doses 200 and 400 mg/Kg. The groups were intraperitoneally administered 3 mg/kg phenobarbital, 200 and 400 mg/kg E. angustifolia’s extract for 2 hours before PTZ injection and 1 ml/kg normal saline for one week (n=6 per group). Seizure induced by administration of PTZ (1%) solution via tail vein of mice. All data were expressed as mean±SEM. One way ANOVA used for statistical evaluation. Statistical significance was accepted at P<0.05. Results: The administration of Elaeagnus angustifolia’s extract at doses 200 and 400 mg/kg was significantly increased Seizure threshold in PTZ-induced mice compared with control group (P <0.001). Conclusion: The hydroethanolic extract of Elaeagnus angustifolia has anticonvulsant effects, possibly through an interaction with inhibitory and excitatory system, antioxidant mechanisms, and ion chennel inhibition.
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Submitted: 15 Apr 2013
Accepted: 16 Jun 2013
ePublished: 30 Apr 2014
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