The Effect Prolonged Exposure to the Low Frequency Electromagnetic Fields on Nitric Oxide Function in the isolated colon of rat

Abstract

Backgrounds and Objectives: Nitric Oxid (NO), as a major signaling molecule in gastrointestinal tract, is produced by esoforms of NO synthase enzyme of arginine possessing, NO has a wide range of physiological and pathophysiological activities like colon cancer. Several studies have implicated that NO is a Key signaling molecule that regulates the processes of tumor genesis; Whereas Electromagnetic Fields (EMF) can affect the NO level in tissues, and according to the increasing use of weak EMF producing devices in homes, hospitals, workplaces, etc. Therefore, the present study was aimed to evaluate the efficacy of the prolonged exposure to the low frequency EMF on NO function in the isolated colon of rat. Material and Methods: In this experimental study, thirty adult male rats were divided into three groups: Experimental group (exposed to 1mT (militesla) and 50Hz for 140), Sham group (kept in the same condition but solenoid off) and control group (kept in normal condition). After 140 days, the mechanical response of longitudinal isolated strips of the isolated colon to L-NAME, were recorded using Power Lab-AtoD system. Results: Despite of few changes about the mechanical response of tissue in the experimental group comparing with other groups, no significant difference was obtained (p>0.05). Conclusion: The prolonged exposure to low frequency electromagnetic fields did not changed the nitric oxide level in the colon.

Keywords: low frequency electromagnetic field, nitric oxide, colon