Med J Tabriz Uni Med Sciences Health Services. 2013;35(4): 52-57.
  Abstract View: 267
  PDF Download: 51


Metastasis Inhibition through Down Regulation of PlGF in Gastric Adenocarcinoma (AGS) Cells

Ameneh Sharifi 1, Mansour Aminzadeh Bukani 2, Hassan Akrami 2*, Mohammad Rahbani Nobar 3, Somayeh Havasi 1

1 Department of Genetics, Ahar Branch of Azad University, Ahar, Iran
2 Department of Biology, School of Science, Razi University, Kermanshah, Iran
3 Department of Clinical Biochemistry, School of Medicine, Tabriz University of Medical Science, Tabriz, Iran
*Corresponding Author: Email: hassan_akrami@yahoo.com


Background and Objectives: Placental Growth Factor (PlGF) is a member of Vascular Endothelial Growth Factors (VEGF) family, which can directly act on angiogenesis, tumor cells motility, tumor metastasis, growth and tumor survival. The purpose of this study was to investigate the preventive effect of siRNA on PlGF gene in gastric cancer cells. Materials and Methods: In this experimental study, gastric adenocarcinoma cells (AGS) with 40 picomol concentration of small interfering RNA (siRNA) related to PlGF gene and cellular migration test was carried out, using wound healing model. Results: PlGF gene 24 and 48 hours after of treatment, expression inhibition showed that migration of AGS cells was prevented compared with control condition, but in 72 hours’ time interval, treatment samples showed migration reduction more than control sample. Conclusion: According to these results, reduction of PlGF gene expression may prevents AGS cells migration and metastasis.
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Submitted: 17 Mar 2013
Accepted: 06 May 2013
ePublished: 30 Oct 2013
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