Abstract
Background & Objective: Coagulase Negative Staphylococci (CNS) have been one of the most important causes of nosocmial infections and have been reported from bacteremia, endocarditis, wound and urinary tract infections and the other infections. As the situation has not been determined in Iran, this study designed to evaluate out breaks of CNS in an Iranian hospital by Pulsed field gel electrophoresis (PFGE).
Material and Methods: In this study 211 clinical strains of staphylococci were isolated from patients who have spent more than 48 hours in the related wards and units. The identification was determined by specific tests. In vitro susceptibility of CNS strains was determined by disc diffusion of Kirby- Bauer recommended by American Society of Microbiology methods.
Results: Among these strains, 63 CNS selected that, 50% were Staphylococcus hemolyticus, 25.7% were Staphylococcus epidermidis and 3.6% were Staphylococcus saprophyticus. According to results, the percent of resistance against Ampicillin, Erythromycin, Ofloxacine, Cefalotin, Cloxacillin, Gentamicin, SXT, Tetracycllin and Vancomycin was 91%, 82.5 %, 22.3%, 22.2%, 17.5%, 17.5%, 16.7%, 12%, 0%, respectively.
Conclusion: PFGE of CNS strains by Sma I digestion pattern revealed a significant diversity within S. hemolyticus strains without any epidemic strain.2 epidemic strains were detected within S. epidermidis strains.It is suspected that, the colonization of S.hemolyticus strain has a preventive role against S.epidermidis outbreaks in hospitals. Although the behavior of S.hemolyticus strains in outbreaks is not obvious.