Abstract
Background and Objectives: It has been reported that elevated level of lipid per oxidation (LPO) in astenozoospermia causes damage of sperms and reduce the fertility. Recently minimum necessity of LPO for the reproducibility of the oocytes and sperms has been reported. In this study effect of high dose of Vit.E and Vit.C on the level of LPO in semen and in vitro effect of the vitamins on the vitality of sperms before and two months after complementary in Astenozoospermia were evaluated.
Material and Methods: The couples refered to infertility ward, 40 normozoospermic fertile males and 60 idiopathic astenozoospermic infertile males were selected using a simple approach. Before treatment, semen samples were collected from the both groups twice and after treatment of the patients(Vit.E 400mg and Vit.C 1000mg per day for two months) two semen samples were collected from the individuals. The levels of the LPO (MDA), Vit.C and E in semen were measured by standard methods and motility and morphology of sperms were determined by who recommendation. Capacitating test of the samples was analyzed using Swim Up method. The ISM1 media culture was used to in vitro investigation of sperms motility.
Results: Significant elevation in the percentage of motile sperms (b degree) was observed in the astenozoospermic males after two months treatment (P<0.05). Treatment with anti oxidant Vitamins without any effect on the concentration and morphology of sperms increased they motility (a and b degree) and vitality. The level of LPO reduced significantly (P<0.001) to normal level after treatment by the anti oxidants.
Conclusion: In this study it was concluded that complementary Vitamins E and C with high dose by reducing the level of LPO increase sperm motility after capacitating test and by increasing the vitality of the cells improve the rate of fertility in vivo and in vitro conditions.