Abstract
Background and Objectives: High capacity of Carrot Seed Ethanol Extract (CSEE) in neutralizing free radicals suggests that the seeds are suitable sources of antioxidants. By in vitro experiments, protective effects of extract seed in peroxidation of membrane lipids, DNA and proteins have been reported. The study designed to evaluate in vivo protective effects of the CSEE on the Gentamicin (Gm) induced oxidative stress in Rats by measuring plasma levels of lipid peroxidation, Paraoxonase(PON1) activities and total antioxidant capacity.
Materials and Methods: Forty male wistar rats were divided into five groups (5 8). They received daily; 0.5ml DMSO (group1, control group), group (2) 0.5ml DMSO containing 200mg/kg extract, group (3) 0.5ml DMSO with 400mg/kg extract, group (4) 0.5ml DMSO containing 400 mg/kg extract plus 5mg/kg Gm and group (5) 0.5ml DMSO with 5mg/kg Gm for 24 daies through Intra Peritoneal injection. The day after last injection 3ml blood samples were collected from the all rats, plasma separated and kept at -70°C.
Lipid peroxidation was determined by measuring plasma levels of MDA using thiobarbituric reaction and oxidative stress was assessed (evaluated) by measuring plasma levels of total antioxidant capacity and activities of PON1 (Aryl esterase) by standard techniques.
Results: The highest and the lowest levels of total antioxidant capacity were noticed in group (3) and group (5) respectively and in groups (2) and (4) comparing with control group, no significant differences were observed. The mean levels of plasma Malone Di Aldehyde (MDA),end product of lipid peroxidation, in groups (1), (3) and (5) were 2.3 0.1,2.1 0.10 and 3.4 0.5 nmol/ml and comparing with other groups the elevation in the group (5) was marked. Significant increase in activity of PON 1 in group (3) and meaningful reduction in group (5) were found while comparing with control no significant changes were observed in those groups (2) and (4).
Conclusion: Treatment of the Rats with CSEE (400mg/kg/day) by increasing the level of total antioxidant capacity and activity of PON1 reduces lipid peroxidation and inhibits the oxidative stress. 1t was concluded that the CSEE may have protective role against toxic and apoptotic agents.