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Med J Tabriz Uni Med Sciences Health Services. 2020;42(1): 73-81.
doi: 10.34172/mj.2020.022
  Abstract View: 1130
  PDF Download: 418

Original Article

A comparative study of hsa-miR-15 gene expression changes in myocardial infarction patients with healthy individuals in Shahid Madani Hospital of Tabriz

Eslam Shiri 1 ORCID logo, Saeid Ghorbian 1* ORCID logo

1 Department of Molecular Genetics, Ahar Branch, Islamic Azad University, Ahar, Iran
*Corresponding Author: *Corresponding author; E-mail:, Email: ghorbian20@yahoo.com

Abstract

Background: miRNA is one of those biomarkers that have different types. Hsa-miR-15 is one of the molecules, which expressed in the heart tissue. MiRNAs are small non-coding RNA that regulates complex cardiac signaling and transcription pathways in cardiac during different condition. The aim of this study was to compare hsa-miR-15 gene expression changes in myocardial infarction patients with healthy individuals.

Methods: In this case-control study, which was included 88 patients with myocardial infarction and 19 healthy individuals who had been referred to the Shahid Madani hospital of Tabriz. After total RNA extraction and cDNA synthesis, the hsa-miR-15 gene expression changes were assessed using Real-time PCR method. For data analysis, we have used independent t-test. Results: The hsa-miR-15 gene expression changes increased in the patients with myocardial infarction compared to healthy individuals. The level of hsa-miR-15 gene expression was shown a statistically significant difference between groups (p >0/05).

Conclusion: The hsa-miR-15gene expression increased in the patient with myocardial infarction and may be used as a biomarker in early diagnosis of myocardial infarction.


How to cite this article: Shiri E, Ghorbian S. [A comparative study of hsa-miR-15 gene expression changes in myocardial infarction patients with healthy individuals in Shahid Madani Hospital of Tabriz]. Med J Tabriz Uni Med Sciences Health Services. 2020 April- May; 42(1):73-81. Persian.
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Submitted: 12 May 2018
ePublished: 26 Feb 2020
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