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Med J Tabriz Uni Med Sciences Health Services. 2019;41(5): 16-24.
doi: 10.34172/mj.2019.054
  Abstract View: 1142
  PDF Download: 390

Original Article

Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR

Fatemeh Ahmadi 1 ORCID logo, Elham Siasi 1* ORCID logo, Kumarss Amini 2 ORCID logo

1 Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran
2 Department of Microbiology, Saveh Branch, Islamic Azad University, Saveh, Iran
*Corresponding Author: *Corresponding author; E-mail: , Email: emi_biotech2006@yahoo.com

Abstract

Background: The genome of Enterococcus has a large number of repetitive sequences that are randomly distributed over DNA. In ERIC-PCR, a separate pattern is obtained for each strain and is considered a separate type. Prokaryotic and eukaryotic genomes include dispersed repeat sequences that are relatively short non-coding, and dispersed in the bacterial genome. BOX-PCR and ERIC-PCR primers are complementary to these repeat sequences and allow for dedicated binding and unique BOX-PCR fingerprint patterns and ERIC-PCR with reproducibility capability.

Methods: In this cross-sectional descriptive study, based on previous studies and 95% confidence level using the formula n = z2P (1-P)/d2 and acceptable error 0.05, total of 60 Enterococcal Faecalis strains were cultured from sterile specimens on KF agar medium and incubated at 37°C for 24h and were identified by biochemical tests of suspected Enterococcal Faecalis. DNA samples were extracted and BOX-PCR and ERIC-PCR tests were performed.

Results: All strains were distinguished in 25 distinct clusters at the level of similarity of 58%. Also, by analyzing the ERIC-PCR results, all strains were segregated into 15 separate clusters at a similar level of 58%.

Conclusion: Molecular fingerprinting with BOX-PCR has a better subtraction and differentiation than ERIC-PCR for typing bacterial isolates and is widely used in epidemiological studies and trace source of infection and taxonomy.


How to cite this article: Ahmadi F, Siasi E, Amini K. [Genomic polymorphism of enterococcus faecalis isolated from clinical cases using two methods of ERIC-PCR and BOX-PCR]. Med J Tabriz Uni Med Sciences Health Services. 2019 December-2020 January; 41(5):16-24. Persian.
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Submitted: 12 Dec 2017
ePublished: 18 Nov 2019
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