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Med J Tabriz Uni Med Sciences. 2021;43(3): 246-253.
doi: 10.34172/mj.2021.050
  Abstract View: 277
  PDF Download: 179

Original Article

Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals

Fatemeh Ganjalishahi 1 ORCID logo, Anahid Hemmatpur 2 ORCID logo, Mahdi Dehghan manshadi 3 ORCID logo, Hossein Hadi nedoushan 3 ORCID logo, Alireza Karimollah 1* ORCID logo

1 Department of Pharmacology, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2 Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3 Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
*Corresponding author; E-mail: karimollah@ssu.ac.ir

Abstract

Background: Paroxetine is one of the well-known antidepressants. Recent studies have focused on paroxetine’s probable immuno-modulatory effects, since findings have indicated inflammation’s role in the pathophysiology of depression. Therefore, in the present study, TLR2 and TLR4 mRNA genes expression was assessed in paroxetine-treated peripheral blood mononuclear cells (PBMCs).

Methods: Venous blood samples were drawn from five healthy men (20-40 years old). Peripheral blood mononuclear cells (PBMCs) were isolated from samples and were cultured. After the first incubation for 24h, phytohemagglutinin plus lipopolysaccharide were added to the cells and then were incubated for 24h. Thereafter, cells were treated with different concentrations of paroxetine in the presence or absence of inhibitors of 5-HT2 and 5-HT7 receptors. After incubation for 48h, RNA was extracted and cDNA was synthesized. Using the real-Time PCR technique, TLR2 and TLR4 genes mRNA expression were evaluated. Statistical analysis of data were carried out using GraphPad Prism 7.

Results: TLR2 and TLR4 mRNA expression were significantly increased in response to paroxetine at all concentrations. Furthermore, the co-culture of cells with the drug and the 5-HT2R and 5HT7R inhibitor simultaneously revealed that paroxetine’s immuno-modulatory effects viaTLR2 are dependent on serotonin, while it is independent of serotonin in the case of TLR4.

Conclusion: Considering paroxetine’s effect in modulating immune responses via increasing TLR2 and TLR4 expression, paroxetine could have therapeutic potentials in diseases with a deficiency in these receptors.

Keywords: Paroxetine, PBMC, TLR2, TLR4
How to cite this article: Ganjalishahi F, Hemmatpour A, Dehghan Manshadi M, Hadi Nedoushan H, Karimollah A. [Paroxetine effect on expression of TLR2 and TLR4 genes in PBMCs of mentally normal individuals]. Med J Tabriz Uni Med Sciences. 2021;43(3):246-253. Persian.
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Submitted: 25 Nov 2019
ePublished: 23 Jun 2021
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