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Med J Tabriz Uni Med Sciences Health Services. 2019;41(1): 72-81.
doi: 10.34172/mj.2019.009
  Abstract View: 731
  PDF Download: 249

Original Article

The effect of 14 weeks of endurance training with two different Intensity on serum irisin level, gene expression of skeletal muscle PGC1-α and FNDC5 and subcutaneous adipose tissue UCP1 in obese rats

Mahbanou Ghaderi 1 ORCID logo, Hamid Mohebbi 2* ORCID logo, Bahram Soltani 3 ORCID logo

1 PhD Student of Exercise Physiology, School of physical Education and Sport Sciences, University of Guilan, Guilan, Iran
2 Department of Exercise Physiology, School of physical Education and Sport Sciences, University of Guilan, Guilan, Iran
3 Department of Pharmacology, School of Medicine, Guilan University of Medical Sciences, Guilan, Iran
*Corresponding Author: *Corresponding author; E-mail: , Email: mohebbi_h@yahoo.com

Abstract

Background: Irisin is a new myokine that has a beneficial effects of exercise on energy metabolism. However, irisin response to different intensities of endurance training is not completely understood.

Methods: In the current experimental study, 18 male Wistar rats were fed a high-fat diet and 6 rats were nourished a standard diet for 12 weeks. After 12 weeks, obese rats were randomly divided into 3 groups: high-intensity training (34 m/min ~85% VO2max), moderate-intensity training (28 m/min ~70% VO2max) and control. Exercise groups trained 5 days/week for 12 weeks on a rodent treadmill. Serum irisin level and gene expression changes were measured using ELISA and Real Time-PCR method, respectively.

Results: In high and moderate-intensity endurance training expression of PGC1-α (P=0.001 and P=0.001), FNDC5 (P=0.013 and P=0.049) and UCP1 (P=0.001 and P=0.001) were significantly more than the control group. Serum irisin level only after high-intensity training was more than the control group (P=0.014). A positive correlation was observed between serum irisin and UCP1 in both of high (P=0.017) and moderate-intensity (P=0.046) training groups.

Conclusion: High-intensity endurance training has a positive effect on the level of serum irisin. Also, there is a positive correlation between irisin and UCP1 gene expression in subcutaneous adipose tissue. However, irisin isn’t the only factor affecting on exercise-induced UCP1 gene expression.


How to cite this article: Gholami Z, Hossein Pourfeizi A, Mahallei M, Farshdousti Hagh M, Movassagpour Akbari A. [Pyruvate Kinase deficiency and its gene mutations in newborns with jaundice in East Azerbaijan province year 2014]. Med J Tabriz Uni Med Sciences Health Services. 2019 April-May;41(1):65-71. Persian.
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Submitted: 21 Apr 2017
ePublished: 05 Mar 2019
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