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Med J Tabriz Uni Med Sciences Health Services. 2020;42(5): 529-536.
doi: 10.34172/mj.2020.076
  Abstract View: 799
  PDF Download: 257

Original Article

The effects of Silibinin on nitrosative stress and matrix metalloproteinase -9 expression in Balb/c mice with breast cancer tumor

Farhad Soleimani 1 ORCID logo, Rasoul Sharifi 2* ORCID logo, Minoo Mahmoodi 3 ORCID logo, Mehrdad Kasaee 4 ORCID logo

1 PhD Student in Biochemistry, Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, Iran
2 Department of Biochemistry, Department of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran
3 Department of Developmental Biology, Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, Iran
4 Department of Biochemistry, Department of Biology, Hamadan Branch, Islamic Azad University, Hamadan, Iran
*Corresponding Author: *Corresponding author; E-mail: , Email: rasoulsharifi.sci@gmail.com

Abstract

Background: Among the factors associated with cancer are the nitrosative stress and increased expression of some Matrix Metalloproteinase (MMP). Silibinin has an anti-tumor effect, therefore, the present study evaluates the effects of slibinin on nitrosative stress indices and Matrix Metalloproteinase -9 (MMP-9) expression in the animal models of breast cancer.

Methods: In this study, 48 Balb/c mice were divided into 6 groups (each group contains 8 mouse): healthy ( control and cancer ) and treatment groups: treatment groups included cancerous mice which receiving 20 mg, 40 mg and 80 mg of silibinin for 3 weeks. In order to induce cancer, 4T1 cell line was used. After enrolling serum and breast tumor samples, NO level and MMP-9 expression in breast tumor biopsy were evaluated. Data were analyzed using one-way ANOVA, Kruskal-Wallis and T-test (p <0.05).

Results: The results revealed that the use 80 mg of silibinin resulted in significantly decreased in NO level in cancerous mice(170.88±15.42μM/ml) compared with untreated cancerous mice(190.38±21.19 μM/ml) (p=0.05). Furthermore, the use of three dose of silibinin led to a non-significant reduction of MMP-9 in the treatment group compared to untreated cancerous mice.

Conclusion: Silibinin treatment has been shown to improve nitrosative stress in breast cancer mice.


How to cite this article: Soleimani F, Sharifi R, Mahmoodi M, Kassaee S.M. [The Effects of Silibinin on Nitrosative Stress and Matrix Metalloproteinase -9 Expression in Balb/c Mice with Breast Cancer Tumor]. Med J Tabriz Uni Med Sciences Health Services. 2020-2021;42(5):529-536. Persian.
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Submitted: 28 Feb 2019
ePublished: 30 Dec 2020
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