Medical Journal of Tabriz University of Medical Sciences and Health Services

Medical Journal of Tabriz University of Medical Sciences and Health Services. 2020;42(3): 245-253.
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Original Article

Inhibition of angiogenesis and improvement of the anti-angiogenic activity of paclitaxel by three insect sex pheromones in chicken chorioallantoic membrane

Sead zeinab payambar pour 1 ORCID logo, Majid Hassanpour-Ezatti 1* ORCID logo, Alireza Askarian Zadeh 2 ORCID logo

1 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2 Department of Plant Protection, Faculty of Agriculture, Shahed University, Tehran, Iran
*Corresponding author; E-mail:


Background: Insect sex pheromones contain long chain polyunsaturated fatty acids. Some types of unsaturated fatty acids have been showed anticancer effects. Angiogenesis is considered as a fundamental process in cancer growth and metastasis. Angiogenesis inhibitory effect of three insect-free pheromones alone and the incremental anti-angiogenic effect of each of them with paclitaxel were evaluated on chorioallantoic membrane (CAM) of chick in the current experimental study.

Methods: Ninety fertilized eggs of Ross race were arranged into 9 groups as follows: control, dexamethasone, paclitaxel, pheromones of currant borer (Synanthedon tipuliformis); leopard moth (Zeuzera pyrina L.) and pistachio twig borer (Kermania pistaciella)-alone, and each sex pheromones plus paclitaxel. On day 8 of chick embryo incubation, 50 µl of each compound were applied to CAMs, and their effects were evaluated on day 9.

Results: Compared with controls, application of each of the pheromones alone is equally effective in reducing vessel length and branching points by 88% and 71%, respectively. Angiogenesis was suppressed (82%) by paclitaxel and stimulated (20%) by dexamethasone. Inhibitory effect of paclitaxel on vascular branching points was further increased (7%) after combination treatment with each of three pheromones. Pheromones of Z. pyrina and K. pistaciella were able to enhance (7%) antiangiogenic effect of paclitaxel.

Conclusion: The direct anti-angiogenic effects of these insect pheromones and the incremental anti-angiogenic effect of each of them with paclitaxel. This antiangiogenic effect seems independent from anti-inflammatory mechanisms. Therefore, we suggest that the effect of these pheromones with paclitaxel be studied on patients with paclitaxel-resistant tumors.

Keywords: Angiogenesis Inhibitors, Chorioallantoic Membrane, Insecta, Pheromones, Paclitaxel, Dexamethasone
How to cite this article: Payambarpour Z S, Hassanpour-Ezatti M, Askarianzadeh A R. [Inhibition of Angiogenesis and Improvement of the Anti-angiogenic Activity of Paclitaxel by Three Insect Sex Pheromones in Chicken Chorioallantoic Membrane]. Med J Tabriz Uni Med Sciences Health Services. 2020 August-September; 42(3):245-253. Persian.
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Submitted: 29 Sep 2018
ePublished: 18 Jul 2020
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