The study of the protective effect of Avicennia marina leaf hydro-alcoholic extract on spermatogenesis and the serum level of testosterone and LH&FSH in male rats induced with carbon tetra chloride

Abstract

Background: Poisoning with tetra carbon chloride produces free radicals in the body tissues including testes. In this research the protective effect of Avicennia marina leaf extract on the rat's spermatogenesis and the serum level of testosterone and LH&FSH induced with carbon tetra chloride are studied. Methods: In this experimental study, 42 rats were divided into six groups including control group(receiving saline normal), sham( receiving olive oil), group induced by CCl4 ( receiving 2ml/kg carbon tetra chloride with 1:1 percent and olive oil featured as single dose), and experiment groups 1, 2, and 3(receiving 2ml/kg carbon tetra chloride with 1:1 percent and olive oil featured as single dose and the extract with daily dosages of 200mg/kg, 400mg/kg, and 800mg/kg). Two hours after injecting carbon tetra chloride, the extract was injected into peritonea once every 24 hours for 96 hours. Results: The normal and disable sperms in the group induced by CCl4 shows a significant reduction (P<0.001). The level of testosterone hormone, FSH and LH in the experiment groups shows a significant increase in comparison to the group induced by CCl4 group (P<0.001). Conclusion: Avicennia marina leaf extract has protective effect against toxic effects of carbon tetra chloride because it contains anti-oxidants.