The effects of diazinon on histomorphometric changes of the number of goblet and the number of epithelial cells of the embryo tissue of the Balb/c mice

Abstract

Background: Diazinon is an organophosphate insecticide that normally used to control different types of harmful insects in agriculture. Methods: 25 laboratory mature female mice divided into 5 equal groups: The control group did not receive diazinon, Sham groups of A and B, respectively, received0.52 and 5.2microliter of emulsifier, Diazinon experimental groups of A and B also received, respectively, 1.3 and 13 microliter form of inhalant. Results: In all histomorphometry and appearance studies, there was no significant difference between the control group and two sham groups but in the experimental group of B there were abnormalities such as atrophy of the fetus and placenta, cutaneous bleeding, the position of fetus was absorbed with extra placenta. The examination of intestinal Histomorphometry of embryos in the average number of goblet cells in a level equal to length of the villi in the experimental group of B in compared to the experimental group of A and the control showed that this increases were not significant. The average number of epithelial cells in a certain level of transverse sections of villi in both the experimental groups compared to control group showed no significant difference. Percentage of goblet cells in the entire villi’s in the experimental group A and B showed a significant decrease in compared to the control group. Conclusion: Consumption of high levels of Diazinon in pregnant mice caused growth and development disorders and physical anomalies in the fetus as well as abnormalities in the development of intestinal tissue of embryos.