Anti-nociceptive effect of arctium lappa l. leafh hydroethanolic extract in male mice

Abstract

Background: Pain is a sign to identify the disease and mostly has a protective effect. Several side effects of chemical drugs caused more likely people are to use herbal treatment. Use of drugs and medicinal plants are common methods of pain control. The aim of this study was to evaluate the antinociceptive effects of Arctiumlappa L. leafh’shydroethanolic extract (AHE) in male mice. Methods: This study contained 72 male mice with the weight of 25±30 gr. Mices were divided randomly in two groups with 6 subgroups (n=6 for each group) including: Control group treated with AHE at doses of 100, 200 and 400mg/kg, morphine (1mg/kg) and case group treated with naloxone (2mg/kg) with dose 200 mg/kg of extract. In order to evaluate the analgesic effect of the extract, writhing and tail flick tests were used. Results: Doses of 200 and 400mg/kg of AHE significantly increased pain threshold compared with the control group in writhing and tail flick tests (P<0.01). Also dose 400mg/kg of AHE have been showed mostly analgesic effect especially in writhing test compared with morphine group (P<0.001). Conclusion: Analgesic effect of the AHE was observed in the tail flick and writhing tests and this analgesic effect of extract probably related to activation of opioid system.