Ali Bagherain
1*, Seyed Hossein Hejazi
2, Motahareh Mirzaei
3, Hamed Mirzaei
4, Hamid Reza Mirzaei
5, Mohammad Jaber Masoud Khoy
61 Department of Biology, School of Agriculture, Islamic Azad University of Saveh, Saveh, Iran
2 Department of Parasitology & Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Departemnt of Biology, School of Basic Sciences, University of Golestan, Gorgan, Iran
4 Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
5 Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6 Department of Horticultural, School of Agriculture & Natural Resources, Islamic Azad University, Abhar Branch, Abhar, Iran
Abstract
Background: Leishmaniasis consisted of a set of parasitic disease with a spectrum of clinical symptoms appear, including coetaneous Leishmaniasis, mucosal and visceral all symptoms. Recently, there has been considerable progress in the use of herbal medicines for the treatment of Leishmaniasis. The purpose of this study was to evaluate the effect of thyme essential oils of four plant species compared with Amphotericin B on Leishmania Amastigotes in invitro.
Methods: Leishmania (Leishmania major) strains were cultured with medium RPMI- 1640 containing 10% fetal calf serum (FCS) and antibiotics and transported at a temperature of 25 ± 2 ° C. The stationary phase of growth were studied in different concentrations of essential oils in comparison with the control drug, Amphotericin B on Leishmania Amastigotes by using colorimetric meth. The absorbance was measured (Optical density) by means of (ELISA reader) and was calculated as the IC 50. The mean absorbance of the groups studied showed no significant difference (P <0/001), the Duncan test showed significant differences between the concentrations of the test and control groups.
Results: The results showed that optical absorption and IC 50 species of thyme essential oils affect drug compared with controls.
Conclusions: Our results showed that the herb thyme is effective for the treatment of coetaneous Leishmaniasis. According to these results suggest that the efficacy was evaluated alone or in combination against human coetaneous Leishmaniasis as a randomized clinical trial.