The Changes of Some Antioxidant Enzymes Activity after Six-Week of Glycogen Depletion Training with Glucose and Glutamine Supplementation in Healthy Nonactive Males

Abstract

Background & Objectives: The aim of the present study was to investigate the effect of glucose and glutamine supplementation on two erythrocyte antioxidants enzymes, glutathione peroxidase and glutathione reductase activity in nonactive men during six-week glycogen depletion training. Materials and Methods: For this purpose, 24 non-athlete healthy men were selected and randomly divided into four groups including: glucose supplementation with glycogen depletion training group (n=6), glutamine supplementation with glycogen depletion training group (n=6), placebo with glycogen depletion training group (n=6) and a control group (n=6) without any treatment. The blood samples were collected from anticubital venous at the onset of training protocol period and 48 hours after final training session. For determining significant between groups One-way ANOVA, compeleted with Tukey post-hoc test. The significant level was chosen as ∝≤0/05. Results: The results showed there was significant increases in glutathione peroxidase enzyme activity of erythrocyte (p<0.05). Post-hoc test showed the significant difference between glucose- traine with placebo-traine and control groups (p<0.001), glutamine-traine with placebo-traine and control groups (p<0.01) and placebo-traine group with control group (p<0.001). Also, there was significant increases in glutathione reductase enzyme activity of erythrocyte (p<0.001). Post-hoc test showed the significant difference between , glutamine-traine with placebo-traine group (p<0.05) and glutamine-traine with control group (p<0.001) and glucose- traine with control group (p<0.001). Conclusion: In summary, the results suggested that glucose and glutamine supplementation cause adaptation erythrocyte glutathione peroxidase and glutathione reductase enzymes activity in glycogen depletion training condition. Therefore, ingestion glucose or glutamine supplementation cause decrease harmful effects free radicals and increase in anti-oxidatives enzymes, during longterm activities.