Logo-mj
Submitted: 12 Feb 2024
Revision: 07 May 2024
Accepted: 12 May 2024
ePublished: 28 Apr 2025
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)

Med J Tabriz Uni Med Sciences. Inpress.
doi: 10.34172/mj.025.33551
  Abstract View: 17

Physiology

Original Article

Melatonin Reduces Inflammation in the Lung Tissue of Type 1 Diabetic Mice Through the ERK/NF-κB Signaling Pathway

Jafar Rezaie 1 ORCID logo, Reza Abbasi 2, Afshin Rahbarghazi 3, Mahdi Ahmaadi 4,5* ORCID logo

1 Solid Tumor Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical Sciences, Urmia, Iran
2 Department of Biology, Urmia University, Urmia, Iran
3 Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4 Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
5 Sarab Faculty of Medical Sciences, Sarab, Iran
*Corresponding Author: Email: mahdi59866@gmail.com

Abstract

Background. Diabetes is associated with inflammation. In this study, the effect of melatonin on the expression of inflammatory genes and the extracellular signal-related kinase/nuclear factor-kappaB (ERK/NF-κB) signaling pathway was investigated in the lung tissue of type 1 diabetic mice.

Methods. Adult male mice weighing 25‒30 g were randomly divided into 4 groups of 10, including control (C), control + melatonin (CM), diabetic (D), and diabetic + melatonin (DM). Streptozotocin (50 mg/kg) was used to induce diabetes. The tumor necrosis factor-alpha (TNF-α) protein level in bronchoalveolar lavage fluid (BALF) was measured by the ELISA test, and the expression of IL-1β, ERK, and NF-κB genes was calculated using the real-time polymerase chain reaction technique.

Results. The findings showed that the concentration of the TNF-α protein in the BALF of groups D and DM was significantly higher than that in group C (P<0.001). The amount of this protein in the DM group significantly decreased compared to the D group (P< 0.05). The expression levels of ERK, IL-1β, and NF-κB genes in the lung tissue of the D and DM groups increased significantly compared to the C and CM groups (P< 0.001). However, the expression levels of these genes in the DM group significantly decreased compared to the D group (P< 0.05, P < 0.001, P < 0.01).

Conclusion. The results of the present study revealed that melatonin inhibited the expression of inflammatory markers and the ERK/NF-κB signaling pathway in the lung tissue of diabetic mice.

Practical Implications. It seems that the induction of diabetes caused an increase in inflammation in the lung tissue of mice, and treatment with melatonin reduced inflammation.


How to cite this article: Rezaie J, Abbasi R, Rahbarghazi A, Ahmadi. Melatonin Reduces Inflammation in the Lung Tissue of Type 1 Diabetic Mice Through the ERK/NF-κB Signaling Pathway. Med J Tabriz Uni Med Sciences. 2025;47(2): doi: 10.34172/mj.025.33551. Persian.
First Name
Last Name
Email Address
Comments
Security code


Abstract View: 18

Your browser does not support the canvas element.


PDF Download: 0

Your browser does not support the canvas element.