Abstract
Background and objectives: Fibroblasts of periodontal Ligaments (PDL-F) have a critical role in periodontal regeneration. it has been reveled that phenytoin induces cell proliferation in gingival fibroblasts (GF). We hypothesized that if phenytoin shows the same effects on PDL-F, it will be considered as a Potentially effective mean for periodontal regeneration. This study was aimed to evaluate the effects of phenytoin on GFS and PDL- Fs.
Materials and methods: A total of ten wistar rat were used as a source of fibroblasts. Gingival tissue was harvested from the papilla of the maxillary central incisors and periodontal ligament was obtained from the mid portion of the extracted lower incisors. After culturing the fibroblasts in a suitable medium, they were assigned to the test and control groups. In the test group, phenytoin was added to medium and the controls left untreated. After 48 houres, The Proliferation fibroblasts were evaluated by an ELISA method (wst-1 and proliferation of both PDL-F and GF were analyzed by independent sample t-test.
Results: phenytoin had a significant proliferative effects on GF. (p<0.05). However, it induced proliferative effects on PDLF rather than GF. On the other hand the comparison of effects of phenytoin on PDLF and GF were significant (p<0.001).
Conclusion: It can be concluded that, phenytoin reveals the same proliferative effects on GFs, in vitro as in vivo and this might be induced on PDL-Fs too.