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Med J Tabriz Uni Med Sciences Health Services. 2019;41(3): 38-43.
doi: 10.34172/mj.2019.031
  Abstract View: 644
  PDF Download: 197

Original Article

Study of rs2858060 polymorphism in Lnc-Ang362 long non-coding sequence as an informative marker in screening of atherosclerotic coronary artery diseases: pilot study

Khadijeh Bazooei 1 ORCID logo, Mahboobeh Nasiri 1* ORCID logo, Hajar Kamfiroozi 2 ORCID logo

1 Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran
2 Cardiologist, Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
*Corresponding Author: *Corresponding author; E-mail: , Email: nasiri@iaua.ac.ir

Abstract

Background: Atherosclerotic coronary artery disease (CAD) is the major cause of death and disability by affecting more than 20% of the individuals over age 35. Lnc-Ang362, an AngII-upregulated long non-coding RNA transcript, regulates the vascular smooth muscle cell proliferation by targeting miR-221 and miR-222. The present study aimed to evaluate the association of the rs2858060 polymorphism in Lnc-Ang362 transcript with the risk of the atherosclerotic CAD.

Methods: This case-control study included 150 affected individuals with CAD and 149 healthy controls. T-ARMS PCR method was used to determine the genotypes of each sample for the polymorphic marker rs2858060 in Lnc-Ang362 gene.

Results: The frequency of the homozygote genotype CC in control group (42.3%) was higher than patient group (31.3%). Individuals with CC genotype were shown to have lower risk of CAD (OR: 0.61; 95%CI: 0.36- 1.01; p= 0.05). The frequency of the polymorphic allele C was 0.51% in control group and 0.43% in patients (OR: 0.68; 95%CI: 0.49- 0.94; p= 0.02).

Conclusion: The long non-coding RNA transcript Lnc-Ang362 may involve in the pathogenesis of the CAD and could consider as a molecular target for prognosis and screening of the patients with CAD.


How to cite this article: Bazooei Kh, Nasiri M, Kamfiroozi H. [Study of rs2858060 polymorphism in Lnc-Ang362 long non-coding sequence as an informative marker in screening of atherosclerotic coronary artery diseases: pilot study]. Med J Tabriz Uni Med Sciences Health Services. 2019 August-September; 41(3):38-43. Persian.
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Submitted: 26 May 2018
Accepted: 24 Jul 2018
ePublished: 04 Jul 2019
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