The effect of aerobic training on VEGF expression in diabetic rats

Abstract

Background: Aerobic training is a well-supported as a useful component of an exercise. One of the most common adaptations that occur in this type of training is creating new vessels and increased angiogenesis factors. Aerobic training can be effective on angiogenesis. The aim of this study was to investigate the effect of aerobic training on (Vascular Endothelial Growth Factor, VEGF) expression in diabetic rats. Methods: 40 Rats divided to two categories equally, diabetic and non-diabetics rats. In case group, diabetes was created through a combination of streptozotocin (STZ) and high-fat meal. Then each category of diabetic and non-diabetic animals will be divided into two groups: \ training (6 days per week on a motorized treadmill) and non-training. At first, the rat began for 10 minutes a day and at a speed of 10 meters per minute, with a slope of 10%.Speed and duration of the exercise, gradually increased, until the duration and intensity of exercise respectively to arrive 1 hour and 26 meters per minute. 48 hours after completion of training period, Cardiac muscle will be removed and immediately placed into liquid nitrogen. Cardiac protein expression of VEGF investigated with method of Elisa. For data analysis, One Way Anova and tukey test will be used to find differences between groups. Results: Diabetes significantly decreased cardiac expression of VEGF protein level (control group: 480.67±14.77 compared with diabetic group: 360.12±33.35; P<0.001). Also, 8 weeks of aerobic training significantly increased cardiac VEGF protein level in healthy (control group: 480.67±14.77 compared with training group: 621.4±34.06; P<0.001) and diabetic rats (diabetic group: 360.12±33.35 compared with diabetic training group: 471.88±25.55; P<0.001). Conclusion: It seems aerobic training can via angiogenic path incurred diabetes improvement. Also, as for results of yielded from this study and kindle regulatory processes by VEGF that via aerobic training be impressed, it is get worth strategy that it can lead in diabetes on development of new therapy methods.