Decreased activity of matrix metalloproteinases in gastric cancer stem cells by treatment with ibuprofen

Abstract

Background: Metastasis is the main cause of cancer-related death. Matrix metalloproteinases (MMPs) play an important role in tumor migration and are known as the main factors in metastasis. Cancer stem cells are a rare population of tumor cells responsible for initiation, spreading and growth of cancer. Ibuprofen, a member of nonsteroidal anti-inflammatory drugs (NSAIDs) family is used for the prevention and treatment of certain cancers. In the present study, we investigate the effect of ibuprofen on metalloproteinase activity in gastric cancer cells. Methods: Gastric cancer stem cell of MKN45 cell lines was isolated by the spheroid colony formation technique. Gastric cancer stem cells were treated with various concentrations of ibuprofen to achieve the IC50 values. MMP activity in Gastric cancer stem cells treated with ibuprofen compared to control cells was investigated by zymography technique. Results: Metalloproteinase activity was reduced in the cancer stem cells treated with ibuprofen in comparison with the control cancer stem cells. Conclusions: Ibuprofen inhibits metastatic ability of gastric cancer cells by reducing metalloproteinase activity in gastric cancer stem cells.