Phenotypic and molecular detection of esbl-producing genes tem-1 and shv-1 in uropathogenic escherichia coli isolates

Abstract

Background: Spread of pathogenic strains carrying genes Extended-spectrum beta-lactamases (ESBL) is one of the major health concerns in the world. These genes caused due to the inefficient beta-lactam antibiotics in the treatment of infectious diseases. The purpose of this study was to determine the prevalence of TEM-1 and SHV-1 beta-lactamase genes in uropathogenic E. coli isolates from the city of Khorramabad. Methods: A total of 100 E. coli isolates were collected from patients with urinary tract infections in clinical laboratories from city of Khorramabad. Originality isolates were confirmed by biochemical tests. ESBLs positives isolates were determined by using a combined disk based instruction the Clinical and Laboratory Standards Institute (CLSI) and then all the positive isolates were studied by PCR assay for the presence of genes TEM-1 and SHV-1. Results: Thirty one (31%) out of 100 E. coli isolates were ESBL positive based on the results of combined disc tests. PCR analysis using the specific primers revealed that 18 isolates (58.06%) contained β–lactamases genes encoding TEM, and while none of isolates did not carry SHV gene. Conclusion: The relatively high prevalence of resistance genes to third generation of cephalosporins among the E. coli isolates was found which indicates a challenge in successful treatment with current antibiotics.