Mohammad reza Nafisi
1, Mohammad Alipour Shadbad
2*, Gorban ali Rahimian
3, Ali Karimi
11 Research Center of Cell & Molecular Science, Sharekord University of Medical Science, Sharekord, Iran
2 Research Center of Molecular Science, Baqiatallah University of Medical Science, Tehran, Iran
3 Department of Internal Disease, Sharekord University of Medical Science, Sharekord, Iran
Abstract
Background & Objectives: Helicobacter pylori prevalence is high in developing countries. This microorganism is accepted as the most important agent of gastritis and as a risk factor for peptic ulcer disease and gastric adenocarcinoma. Currently many diagnostic methods exist for detecting H. pylori, however they all have limitations. The aim of this descriptive study was to evaluate diagnostic methods, such as rapid urease test, culture and polymerase chain reaction (PCR).
Materials and Methods: For identification of Helicobacter pylori 100 patients (46 women and 54 men) who were suffering from digestive complaints and referred to the endoscopy department of Hajar Hospital in Sharkord were participated in the study. Gastric biopsy samples were collected from each patient, then polymerase chain reaction, culture and rapid urease test were performed. DNA extraction was followed by PCR was used for diagnosis of ureC gene.
Results: Biopsy samples from100 patients were evaluated. H. pylori was positively identified by PCR in 78/100 (78%) of the patients, while positive samples were found in 52 (52%) and 48 (48%) of the patients by RUT and culture methods, respectively. In results of the culture, there was an agreement of 100% with PCR.
Conclusion: Three different methods for the detection of Helicobacter pylori were evaluated. Given that the PCR test has higher sensitivity and specificity to detect H.pylori comparing rapid urea as test, therefore this method could be used to detect H.pylori.