Nanotechnology is the New Method for Rapid Detection of Vibrio Cholerae

Abstract

Backgrounds and Objectives: Rapid identification of Vibrio cholera is important in epidemics and bioterrorism wars. On the other hand, the conventional method is time consuming and costly. The aim of this study was to develop a rapid, inexpensive and high sensitive method based on magnetic nanoparticles for trapping bacterial DNA. Materials and Methods: In this study, biotinalated probes were used for trapping Vibrio cholerae DNA. Finally designed primers were used to detect bacteria. Results: According to PCR identified results showed that only Vibrio cholera were identified, and; concluded that the biotinalated probe is specific for bacterium Vibrio choleraecholera. Conclusions: According to the findings of this study, Sensitive and specific detection of the bacterium Vibrio cholerae is assessed and validated with this method efficiently. And in terms of time and at lowest costs are introduced an efficient method.