Tabriz University of Medical Sciences Medical Journal of Tabriz University of Medical Sciences 2783-2031 36 4 2014 10 05 The Effect of Plgf Gene Knockdown on Enzymes Metalloproteinase-2 and -9 (MMP-2&-9) Activity 10 13 EN Hassan Akrami Mansour Aminzadeh Ameneh Sharifi Journal Article 2013 09 24 2013 12 15 Background and Objectives: Placental Growth Factor (PlGF) is a member of Vascular Endothelial Growth Factors (VEGF) family, which is involved in several pathologic processes, including the growth and spread of malignancies. PlGF promote cancer metastasis by controlling the MMP (Matrix metalloproteinase) activity. In the present study, we investigate the correlation between the expression of PlGF and matrix metalloproteinase enzymes activity in human Adenocarcinoma Gastric Cell (AGC). Methods and Materials: Human AGC were treated with 20, 40 and 80 picomol concentrations small interfering RNA (siRNA) related to PlGF mRNA for24, 48 and 72 hours. Then MMP-2 and MMP-9 enzymatic activity was measured by gelatin zymography test. Results: The gelatin zymography results indicated that the activity of MMP2&9 was fully inhibited in AGC which were treated with various concentrations of PlGF-siRNA for 24 hours. Our results also showed that in cells were treated with PlGF-siRNA for 48 hours the activity of MMP2 and MMP 9 were decreased (but not fully inhibited). Conclusion: According to this study reduction of PlGF gene expression may prevents MMP2/9 activity in AGC. These data suggest that PlGF may function up stream of MMP in gastric adenocarcinoma cells. Probably PlGF effect on cancer cell metastasis via regulation activity of MMPs.